Basic Points That You Need to Consider When You Are About to Replace Your HPLC Column
Sometimes it can make you feel frustrated when you discover that even after running tests that you obviously know the results will give you something different, it can result to waste of time. Or you may find that you are running a column in a typical method that you have spent months trying to optimize only to realize that the results offered cannot be interpreted data. There are high chances that you have a contaminated HPLC column. Here are some of the diagnostic strategies that you can use when you are looking forward to determining how you can be able to choose the right HPLC column to ensure that you are safe all the time.
First you need to ensure if the column has been washed by the last user. In many cases the reversed-phase columns are typically stored in an organic solvent that is miscible like methanol-water. You then need to switch to a running mobile phase and ensure that you monitor the baseline closely for the flow. It is essential that you note that when an organic solvent is added to the mobile phase, it will be effortless to re-equilibrate with the column.
You need to know that having procedural ways of handling your clogged HPLC column is very important, you need to carry out some troubleshooting to resolve the issue. You may either choose to use a dilution of the sample or just use an external solubility to see if it dissolves. There is need to know that for you to be able to have an easy time, ensure that you get to know how you can try more concentrated solvents to remove these parts as they have been seen to have a significant impact this time around.
Before you make a conclusion to replace the HPLC column, you need to first clean with a strong solvent that is compatible with your mobile phase. Be sure that you also try to flush the materials using the reverse method, it can result to interfering the bed integrity, and this can, on the other hand, lead to tempering the performance, and it matters so much.
You have seen that all the issues that we have discussed will have a negative impact on the performance of your HPLC column, take time in carrying out various kinds tests so that you ensure that you enjoy efficient results that will ensure that you feature with ease. If you find that the practices are not working through the troubleshooting it is time to replace your column, you will be able to boost better experiments in your laboratory.